Findings With FT819 Demonstrate Promising, Accessible Approach to CAR T-Cell Therapy

Bob Valamehr, PhD

Findings, presented at the 2018 AACR Annual Meeting, showed that an off-the-shelf, dual-targeted chimeric antigen receptor (CAR) T-cell approach demonstrated positive results in preclinical specificity, functionality, and efficacy studies.

FT819 is an off-the-shelf, T-cell receptor (TCR)-less CD19-targeted CAR T-cell product.

“This product is responsive, cytotoxic, and specific,” noted Bob Valamehr, PhD, vice president of cancer immunotherapy at Fate Therapeutics Inc, the manufacturer of the drug.

There is hope that this product could potentially be more accessible than conventional CAR T-cell therapies, which tend to be incredibly personalized, time-consuming to produce, and include only enough cells for a single-dose treatment with variable quality.

Investigators begin with cells from a healthy donor rather than the patient in this new approach, where they combine reprogramming of peripheral blood-derived T cells with targeted insertion of a CD19 CAR into the T-cell receptor α (TRAC) locus under the transcriptional control of its endogenous regulatory elements to generate a single cell-derived clonal TRAC-targeted CAR-expressing master pluripotent stem cell (iPSC) line to generate FT819.

“Our off-the-shelf vision starts with an iPSC—a pluripotent cell that has the unique ability of unlimited self-renewal and the capacity to differentiate in to 200 cell types. Using this cell, we can effectively place multiple attributes in to a cell at the single cell level and create a master cell bank,” Valamehr added.

The iPSC line for the production of CAR T-cells provides distinct advantages over autologous and allogeneic approaches, in the process of creating the master line cell. The investigators are then able to produce large quantities of what Valamehr called “universal CAR19 T cells” which are not patient-restricted.

“This master cell bank, rising from a single-cell iPSC clonal population, can be plugged in to our proprietary differentiation and expansion strategy to make a mass number of T cells that are capable of multi-dose application and can basically begin to address the many challenges as discussed earlier—including creation of a homogenous product, at one time different reprogramming strategy and engineering strategy, a cost-effective process, and starting with material that is from a healthy starting source."

By using donor T cells, they can attack the patient’s tissues and organs, potentially resulting in graft-versus-host disease (GVHD), making it critical to deactivate or remove TCR.

The iPSC line dependably converted into CD34-positive cells during the stage-specific differentiation, which were then differentiated towards CD8-positive cells with uniform CAR expression (95 +/- 5%) during the absence of TCR expression, then eliminating the likelihood of GVHD.

“The product is pure for CAR expression and has complete elimination of TCR,” Valamehr added. "As we have been told through several clinical trials, even few cells expressing TCR can elicit GVHD. So, this pure product is now safe and highly efficacious."

When challenged with CD19-positive tumor cells by producing cytokines (IFN-gamma, TNF-alpha, and interleukin-2) and mediators of cell death (CD107a/b, perforin, and granzyme B), in vitro functional studies showed that FT819 displayed an efficient cytotoxic T-cell response. Additionally, FT819 was found to be target specific by attacking only CD19-positive tumor cells and sparing the CD19-negative tumor cells.

A second targeting receptor was also found in FT819, designed to broaden the therapy’s efficacy: a CD16 Fc receptor that can bind to tumor cells coated with antibodies, which enables the agent to be administered in combination with other proven cancer treatments, like monoclonal antibodies targeting CD20-positive tumor cells, to potentially overcome tumor antigen escape.

FT819 was also shown to elicit antibody-dependent cell-mediated cytotoxicity when combined with a therapeutic antibody targeting CD20, all through the expression of CD16 Fc receptor, in the in vitro studies.

Valamehr said that the cells can be can be packaged, stored, and made readily available for treatment for a large number of patients.

“CAR T cells can be robustly manufactured from a multi-point engineered master iPSC line. The master iPSC line is self-renewing and can be used repeatedly without sourcing new donor material or re-engineering cells. A single manufacturing campaign yields large quantities of CAR T cells and can be repeated in the same exact manner,” he added.

“This master cell bank, rising from a single-cell iPSC clonal population, can be plugged in to our proprietary differentiation and expansion strategy to make a mass number of T cells that are capable of multi-dose application and can basically begin to address the many challenges as discussed earlier—including creation of a homogenous product, at one time different reprogramming strategy and engineering strategy, a cost-effective process, and starting with material that is from a healthy starting source."

Valamehr hopes the product will be evaluated in investigational new drug studies in the coming year, though it is still in the preclinical stages currently.

“FT819 is an off-the-shelf CAR T-cell therapy with the potential to improve safety and efficacy, address antigen escape, broaden patient accessibility, and reduce cost of manufacture delivery, and really begin to make this available to the masses," he added.

Alice T. Shaw, MD, PhD, moderator of the AACR press conference, co-chair of the AACR Annual Meeting Clinical Trials Committee, and director of the Center for Thoracic Cancers at Massachusetts General Hospital Cancer Center, acknowledged the promising results that this product seems to have in treating a large number of patients.

“This is very exciting and highlights the potential utility of renewable engineered pluripotent stem cells to generate large quantities of these universal CAR T cells,” Shaw said. “There are not only practical advantages to this approach, making the therapy more accessible to patients, but there are also important biological and therapeutic advantages in terms of our ability to modify these CAR T cells.”

Reference:

Peralta E, Hardy I, Ortiz E, et al. Generation of off-the-shelf TCR-less CAR-targeted cytotoxic T cells from renewable pluripotent cells for cancer immunotherapy. In: Proceedings from the 2018 AACR Annual Meeting; April 14-18, 2018; Chicago, Illinois. Abstract LB-108.